High-Content Analysis
The Screening Facility couples genetic and chemical high throughput screening with multiparametric phenotypic image analysis to support translational cancer research. We employ functional genomics (siRNA, shRNA, CRISPR) tools in both pooled and arrayed approaches to support target identification and target validation in multiple types of cancer. Our state-of-the-art image analysis capabilities, including a machine learning module, support identification of a suite of cellular phenotypes in both 2D and 3D environments.
Our Perkin Elmer Opera High-Content Screening system is supported by a robotic plate-loading arm, and JANUS and Wellmate liquid handling systems.
High-Content Analysis Equipment
Opera Phenix
The Opera Phenix is a high-throughput confocal microscope primarily used for high-content screening and phenotype analysis. The system supports automated plate loading and image acquisition. The Opera Phenix is particulary advanced in 3D imaging of cells, organoids, spheroids and tissues. Coupled to Harmony/Columbus analysis software, the Opera Phenix can generate large amounts of data suitable for bioinformatics and machine learning.
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Emily Kay |
Jessica Perochon |
Perkin Elmer Janus MDT and Varispan
The Janus is an automated liquid handler with integrated robotics in a clean-air cabinet. Using the MDT fixed heads, 96 well and 384 well aspirate/dispense operations can be carried out. It is most commonly used to replicate plates, in high-throughput CRISPR, siRNA and compound screens. The Varispan platform can perform a versatile range of pre-programmed individual well operations, such as serial dilutions and cherry-picking of compounds from larger libraries.
Formulatrix Mantis
The Mantis is a liquid handling robot that uses microfluidics to dispense volumes from 0.1 – 5ul (and multiples), accurately and precisely with little waste. It is capable of complex, programmed, multiple liquid dispenses, and is commonly used to dispense cells in matrices, such are Matrigel, for 3D, high-throughput and high-content assays.
High-Content Analysis Team
Lynn McGarryHigh Content Screening |
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Ryan CorbynImage Analysis |
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